RNAseq Differential Expression Analysis
نویسنده
چکیده
Dataset taken from Somel et al. 2012: (check publication for details) • 3 human + 3 chimp brain transcriptome data • Illumina RNA-Seq, paired-end 76bp • Reads mapped to human and chimp genome and reads counted for each transcript Download the following data files into a directory of your choice (e.g. data): • Human: http://www.nowick-lab.info/wp-content/uploads/2013/12/segemehl.hg19.readCount.txt • Chimp: http://www.nowick-lab.info/wp-content/uploads/2013/12/segemehl.panTro3.readCount.txt Read dataset into R # filenames HreadCounts <"data/segemehl.hg19.readCount.txt" CreadCounts <"data/segemehl.panTro3.readCount.txt" # counts Hcounts <read.table(HreadCounts, head=T, sep="\t", quote="", stringsAsFactor=FALSE, row.names="id") Ccounts <read.table(CreadCounts, head=T, sep="\t", quote="", stringsAsFactor=FALSE, row.names="id")
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